人胰腺癌组织中PFKFB3表达及其与MVD相关性的研究

目的:检测人胰腺癌组织6磷酸果糖激酶2/果糖双磷酸酶2同工酶3(PFKFB3)的表达及微血管密度(MVD),并探讨二者临床意义和相关性。方法:采用免疫组化SP法检测41例胰腺癌组织和11例癌旁组织石蜡块中PFKFB3的表达情况,并对CD34标记阳性的血管进行微血管密度计数,分析PFKFB3、MVD与胰腺癌临床病理特征的相关性及二者之间的相关性。结果:胰腺癌组织和癌旁组织中PFKFB3的阳性表达率分别为63.41%和9.09%,胰腺癌组织显著高于癌旁组织(P0.01);PFKFB3呈阳性和阴性表达的胰腺癌组织MVD值分别为(49.12±12.04)、(35.40±8.12),两者比较差异有统计学意义(P0.01)。PFKFB3表达与胰腺癌的分化程度显著相关(P=0.035),MVD与胰腺癌TNM分期及淋巴结转移显著相关(P0.05)。Spearman等级相关分析显示:胰腺癌PFKFB3表达与MVD值呈显著正相关(r=0.551,P0.01)。结论:胰腺癌组织中PFKFB3呈高表达,可能通过促进血管生成,在胰腺癌的发生发展、转移过程中发挥重要作用,并可能作为胰腺癌预防、治疗和预后评估的参考指标。

Study on the PFKFB3 Expression in Pancreatic Cancer and the Correlation with Microvessel Density

Objective:To detect the expression of PFKFB3 and microvessel density (MVD) in human pancreatic cancer tissues and investigate the clinical significance and correlation of PFKFB3 with MVD.Methods:The expressions of PFKFB3 in 41 cases of pancreatic cancer and 11 cases of para-carcinoma tissues were detected by immunohistochemical SP method and CD34 positive vessels considered as MVD in those tissues were counted. Then relationships between expression of PFKFB3 and MVD, correlation of PFKFB3 with clinical characteristics, MVD with clinical characteristics were analyzed respectively.Results:The positive expression rate of PFKFB3 in pancreatic tissue and para-carcinoma tissue were 63.41% and 9.09%, which was significantly higher in the pancreatic tissue (P<0.01); the MVD of pancreatic tissue with positive and negative PFKFB3 expression were (49.12± 12.04), (35.40± 8.12) respectively (P<0.01). The expression of PFKFB3 was significantly related to the differentiation degree of pancreatic tissue (P=0.035), MVD was related to both TNMstage and lymph node metastasis of pancreatic tissue (P<0.05). Spearman correlation analysis showed that the expression of PFKFB3 was positively correlated with MVD(r=0.551, P<0.01).Conclusion:The up-regulation of PFKFB3 expression may play an important role in the tumor genesis and development of pancreatic cancer through promoting the angiogenesis.PFKFB3 could be used as a reference index of the prevention, treatment and prognostic prediction of pancreatic cancer tissue.