Modification of replicon operation in HeLa cells by 2,4-dinitrophenol |
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Authors: | J R Gautschi R M Kern R B Painter |
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Institution: | Laboratory of Radiobiology University of California San Francisco, Calif. 94143, U.S.A. |
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Abstract: | Cycloheximide causes inhibition of semiconservative DNA replication in HeLa cells by reducing the average rate of DNA chain elongation. 2,4-Dinitrophenol inhibits semiconservative DNA replication (50 to 80% inhibitions at 10?3 to 5 × 10?3 M-2,4-dinitrophenol) without affecting the average rate of DNA chain elongation. Therefore, at any given time the number of replicating sections of DNA per DNA-synthesizing (S-phase) cell appears to be reduced in the presence of 2,4-dinitrophenol.Radioactivity profiles of pulse-labeled DNA in alkaline sucrose gradients suggest that 2,4-dinitrophenol modifies initiation and termination patterns of replicating sections, most of which are found to be 10 to 80 μm (mode: 15 to 30 μm) under control conditions. DNA synthesized in the presence of 2,4-dinitrophenol has the density of control DNA, is metabolically stable, and after mitosis, functions normally as a template in the next round of replication. |
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Keywords: | Dnp 2 4-dinitrophenol BrdUrd bromodeoxyuridine dThd thymidine |
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