Cytokine-induced selective increase of high-molecular-weight bFGF isoforms and their subcellular kinetics in cultured rat hippocampal astrocytes |
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Authors: | Hiroyuki Kamiguchi Kazunari Yoshida Hirooki Wakamoto Makoto Inaba Hikaru Sasaki Mitsuhiro Otani Shigeo Toya |
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Institution: | (1) Department of Neurosurgery, School of Medicine, Kein University, 35 Shinanomachi, Shinjuku-ku, 160 Tokyo, Japan |
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Abstract: | Cytokines such as interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) and epidermal growth factor (EGF) are probable factors
responsible for up-regulation of basic fibroblast growth factor (bFGF) expression in reactive astrocytes following brain damage,
however the effect of these cytokines on the expression of each bFGF-isoform has not been elucidated. Western biot analysis
revealed the expression of 18, 22 and 24-kD bFGF isoforms in cultured rat hippocampal astrocytes, and the expression of high
molecular weight (HMW)-isoforms (22 and 24-kD isoforms) but not of 18-kD isoform was selectively increased by cytokines. Immunofluorescent
analysis demonstrated that bFGF content in the cytoplasm of astrocytes is initially increased by cytokines followed by nuclear
targeting and localization in agreement with the previous evidence that HMW-isoforms possess a nuclear targeting signal. The
present results suggest the important role of HMW-bFGF isoforms in the response of nervous tissue to injury. |
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Keywords: | Basic fibroblast growth factor isoform astrocyte interleukin-1β tumor necrosis factor-α epidermal growth factor |
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