固定化白腐真菌对多种染料脱色的研究*

白腐真菌F17Schizophyllumsp.对不同结构的多种染料具有较强的脱色能力。聚酯无纺布是该菌固定化的最佳载体。利用正交实验,确定了该菌株固定化细胞制备的最优操作条件。与游离菌相比,固定化菌不仅提高其脱色能力,而且在pH值和染料浓度的变化情况下,仍保持稳定的脱色率。固定化菌对染料脱色后的紫外可见光谱分析表明,可见光区吸收峰消失,紫外区的光吸收峰有所增加,染料结构发生了变化。

STUDY ON DECOLORIZATION OF VARIOUS DYES BY IMMOBILIZED WHITE ROT FUNGUS SCHIZOPHYLLUM SP.

A white rot fungus F17(Schizophyllum sp.) was very effective to decolorize a wide range of structurally diverse dyes. Non-woven fabric is the best immobilized carrier of the strain. Optimal preparation condition of the strain immobilization is determined by the cross-test experiments. Comparing with free cells, immobilization not only enhanced the ability to decolorize dye but also kept the stable rate of decolorization in the condition of different pH values and dye concentrations. The products decolorized by immobilized F17 were determined by UV-VIS spectra assay. The absorption in visible range disappeared,but the absorption in UV range were obviously increased. The result showed that the structures of dyes had changed greatly.