| 在大肠杆菌中反义RNA抑制乙型肝炎病毒核心抗原的表达 |
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| 引用本文： | 赵小侠,毛东丽,张爱臣,侯云德. 在大肠杆菌中反义RNA抑制乙型肝炎病毒核心抗原的表达[J]. 病毒学报, 1992, 0(2) |
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| 作者姓名： | 赵小侠 毛东丽 张爱臣 侯云德 |
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| 作者单位： | 中国预防医学科学院病毒学研究所病毒基因工程国家重点实验室 北京(赵小侠，毛东丽，张爱臣)，中国预防医学科学院病毒学研究所病毒基因工程国家重点实验室 北京(侯云德) |
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| 摘 要： | 将乙型肝炎病毒核心抗原(HBcAg)基因的DNA序列分别按正、反方向置tac启动子的控制下,并克隆进入同一个质粒中,转化大肠杆菌JM103后,观察到HBcAg和HBeAg的合成均明显低于仅含有HBcAg基因的细菌,最大抑制率可达70％～80％,分子杂交可见反义RNA存在。
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| 关 键 词： | 乙型肝炎病毒核心抗原 反义RNA |
INHIBITION OF HBcAg GENE EXPRESSION BY ITS ANTIS ENS ERNA IN E.coli |
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| Zhao Xiaoxia Mao Dongli Zhang Aichen Hou YundeInstitute of Virology,Chinese Academy of Preventive Medicine,National Laboratory of Molecular Virology,Beijing. INHIBITION OF HBcAg GENE EXPRESSION BY ITS ANTIS ENS ERNA IN E.coli[J]. Chinese journal of virology, 1992, 0(2) |
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| Authors: | Zhao Xiaoxia Mao Dongli Zhang Aichen Hou YundeInstitute of Virology Chinese Academy of Preventive Medicine National Laboratory of Molecular Virology Beijing |
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| Abstract: | HBcAg gene DNA sequence was placed under the control of tac promoter in both directions, then cloned into the same plasmid. The synthesis of HBcAg and HBeAg in E.coli transformed with the above plasmids was markedly lower than that in bacteria harboring the plasmid only expressing HBcAg. The inhibitory rate is about 70%-80%. HBcAg anti sense RNA was identified by hybridization reaction. |
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| Keywords: | Hepatitis B viral core antigen Antisense RNA |
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