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Enhancement of nitrogen fixation inAnabaena flos-aquae (Cyanobacteria) via low-dose ultrasonic treatment
Authors:D. A. Francko  S. Al-Hamdani  G. J. Joo
Affiliation:(1) Department of Botany, Miami University, Oxford, OH 45056, USA;(2) Present address: Department of Biology, Jacksonville State University, Jacksonville, AL 36265, USA;(3) Present address: Department of Biology, Pusan National University, Pusan, 609-735, S. Korea
Abstract:Low-dose (50 W), short-duration (5 min) ultrasonic treatments were delivered to log phase batch cultures of the cyanobacteriumAnabaena flos-aquae grown in nitrogen-free medium. Near the mid-point of nine-day experimental periods, ultrasonically-treated cultures exhibited higher growth rates, enhanced chlorophylla content, and greater acetylene reduction capacity when compared to cultures grown with reciprocal shaking alone. Heterocyst frequency was markedly higher in ultrasonically-treated cultures over the duration of five-day experimental periods.Author for correspondence
Keywords:cyanobacteria  Anabaena flos-aquae  ultrasound  nitrogen fixation  heterocyst  algal biotechnology
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