Homocitrate is a component of the iron-molybdenum cofactor of nitrogenase |
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| Authors: | T R Hoover J Imperial P W Ludden V K Shah |
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| Institution: | Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin-Madison 53706. |
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| Abstract: | When apodinitrogenase (lacking FeMo-co) was activated with FeMo-co synthesized in vitro in the presence of 3H-labeled homocitrate, label was incorporated into dinitrogenase. The physical association of the label with FeMo-co was demonstrated by reisolation and purification of the cofactor from dinitrogenase. The presence of homocitrate in FeMo-co was established by NMR analysis of the organic acid extracted from dinitrogenase. Quantitation of homocitrate in dinitrogenase showed it to be present at a 1:1 ratio with molybdenum. |
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