Inheritance of Arabidopsis DNA in offspring from Brassica napus and A. thaliana somatic hybrids |
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Authors: | S Bohman J Forsberg K Glimelius C Dixelius |
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Institution: | (1) Department of Plant Biology, Uppsala Genetic Center, Swedish University of Agricultural Sciences, Box 7080, S-750 07 Uppsala, Sweden Fax: +46 18 673279 E-mail: Svante.Bohman@vbiol.slu.se, SE |
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Abstract: | Chromosome counts and RFLP markers mapped to Arabidopsis thaliana were used to determine the proportion of eliminated chromosomes and retained A. thaliana DNA in the back-crossed (BC) progeny derived from symmetric and asymmetric somatic hybrids between Brassica napus and A. thaliana. All plants were analysed for the presence of two RFLP markers per chromosome, preferably with one located on each chromosome
arm. A reduction in both A. thaliana RFLP markers and chromosome numbers was found in the BC1 and BC2 generations of the symmetric hybrids as well as in the BC1 generation of the asymmetric hybrids. In the symmetric hybrids, two back-crosses to B. napus were required to reduce the frequency of retained A. thaliana loci to 42.4% and mean chromosome number to 39.4. In comparison, the BC1 progeny of the asymmetric hybrids had 16% of the analysed A. thaliana loci present and an average of 38.4 chromosomes maintained. When the frequency of A. thaliana chromosomes with both analysed loci maintained was compared with the frequency of chromosomes with one locus lost and one
kept, a reduction in the number of complete chromosomes between BC1 and BC2 derived from the symmetric hybrids was observed. Among the BC1 plants in the asymmetric group the situation was different, with higher amounts of incomplete donor chromosomes compared
to whole chromosomes. The results indicate that A. thaliana chromosome fragments are more often found in the progeny of irradiated hybrids, while back-crossed symmetric hybrids have
more complete chromosomes.
Received: 2 April 1998 / Accepted: 14 July 1998 |
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Keywords: | Brassicaceae Back-crossed symmetric and asymmetric somatic hybrids RFLP analysis Chromosomes Intergenomic translocation |
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