Comprehensive two-dimensional liquid chromatography mass spectrometric profiling of the rat hippocampal proteome |
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| Authors: | Yang Xiaoping Levin Yishai Rahmoune Hassan Ma Dan Schöffmann Stephanie Umrania Yagnesh Guest Paul C Bahn Sabine |
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| Affiliation: | Cambridge Centre of Neuropsycharitic Research, Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, UK. |
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| Abstract: | In this study, we performed the first high‐throughput and comprehensive proteomic profiling of the rat hippocampal proteome. Using a combination of 2‐D LC‐MS and data analysis with the Rosetta Elucidator® system, we identified 1340 unique proteins. Functional classification showed that most of these were associated with synaptic function and comprised a high proportion of phosphorylated proteins and analytically challenging classes of membrane proteins such as ion channel receptor subunits. |
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| Keywords: | 2‐D liquid chromatography Animal proteomics Hippocampus Mass spectrometry |
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