黑翅痂蝗血细胞形态学的研究

【目的】蝗虫血细胞的研究以及不同血细胞类型间的演化关系,是蝗虫细胞免疫学的依据,也是防蝗治蝗的重要依据。【方法】本研究通过Wright-Giemsa对黑翅痂蝗Bryodema nigroptera Zheng血细胞进行了混合染色,并使用光学显微镜进行了观察。【结果】发现黑翅痂蝗血细胞包括原血胞、浆血胞、粒血胞、类绛色血细胞、囊血胞、脂血胞、蠕形细胞和巨核血细胞8种类型。【结论】在成虫体内,原血胞占细胞总数的1%,浆血胞占32.2%,粒血胞占57.3%,类绛色血细胞占1%,囊血胞占6%,脂血胞占1%,蠕形细胞占1%,巨核细胞占细胞总数的0.5%,粒血胞数量最多,巨核细胞最少。

Morphology of the hemocytes of Bryodema nigroptera Zheng

[Objectives] To describe the characteristics and morphology of the hemocytes of Bryodema nigroptera Zheng and describe the evolutionary relationships between the different types of hemocytes in order to further understanding of the cellular immunology of locusts and provide a basis for locust prevention and control. [Methods] Hemocytes of B. nigroptera were stained with Wright-Giemsa and observed under an optical microscope. [Results] B. nigroptera has 8 types of hemocytes; prohaemocytes, plasmatocytes, granulocytes, oenocytoids, cystocytes, adipohemocytes, vermiform cells and megakaryocytes. [Conclusion] In adults, prohaemocytes accounted for 1%, plasmatocytes for 32.2%, granulocytes for 57.3%, oenocytoids for 1%, Cystocytes for 6%, adipohemocytes for 1%, vermiform cells for 1% and megakaryocytes for 0.5% of all hemocytes. Granulocytes were the most abundant followed by plasmatocytes, and megakaryocytes were the least abundant.