北京地区六种蜜蜂病毒病的流行病学研究

【目的】对蜜蜂的6种病毒:以色列急性麻痹病毒(Israeli acute paralysis virus,IAPV)、残翅病毒(Deformed wing virus,DWV)、囊状幼虫病病毒(Sacbrood virus,SBV)、急性蜜蜂麻痹病毒(Acute bee paralysis virus,ABPV)、黑蜂王台病毒(Black queen cell virus,BQCV)、慢性麻痹病毒(Chronic bee paralysis virus,CBPV)在北京地区的流行情况进行调查,以期为该地区蜜蜂病毒病的防控提供一定的理论依据。【方法】应用多重RT-PCR法确定上述6种病毒在该地区的感染情况,并通过序列分析确定特异性。【结果】在所有检测样本中均未检测到急性麻痹病病毒和慢性麻痹病病毒,感染率最高的是以色列急性麻痹病毒,其次是残翅病毒。检测的样本普遍存在混合感染。【结论】以色列急性麻痹病毒、残翅病毒、囊状幼虫病病毒、黑蜂王台病毒4种病毒可能在北京地区广泛分布。

Occurrence and prevalence of six bee viruses in Beijing

[Objectives] To provide a theoretical basis for the prevention of bee virus diseases in Beijing. [Methods] Six honey bee viruses; Israel acute paralysis virus （iAPV）, deformed wing virus （DWV）, Sacbrood virus （SBV）, acute bee paralysis virus （ABPV）, black queen cell virus （BQCV） and chronic bee paralysis virus （CBPV） were surveyed using multiplex RT-PCR, and the specificity of the RT-PCR assay was confirmed by sequence analysis. [Results] IAPV was the most widespread virus with DWV was the second most prevalent. Chronic bee paralysis virus （CBPV） and acute bee paralysis virus （ABPV） were not detected. Simultaneous multiple infections of viruses were common in the tested samples. [Conclusion] IAPV, DWV, SBV and BQCV are probably wide spread in the Beijing region.