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昆虫触角电位(EAG)及其与气谱联用(GC-EAD)技术
引用本文:黄翠虹,李静静,周琳,闫凤鸣. 昆虫触角电位(EAG)及其与气谱联用(GC-EAD)技术[J]. 昆虫知识, 2014, 0(2): 579-585
作者姓名:黄翠虹  李静静  周琳  闫凤鸣
作者单位:[1]顺德职业技术学院,广东528333 [2]河南农业大学植物保护学院,郑州450002
基金项目:国家公益性行业(农业)科研专项(201203036)
摘    要:
昆虫触角电位(EAG)和气相色谱-触角电位(GC-EAD)是昆虫化学生态学中最重要的电生理技术,在研究昆虫对信息化学物质的感受机理、筛选活性信息素成分或植物次生物质等方面发挥关键作用。本文介绍这两种技术的基本原理、操作步骤和应用实例等,并对使用中应该注意的事项进行了详尽讨论。

关 键 词:昆虫触角电位技术  EAG  气相色谱-触角电位技术  GC-EAD  原理  操作步骤

EAG and GC-EAD techniques
HUANG Cui-Hong,LI Jing-Jing,ZHOU Lin,YAN Feng-Ming. EAG and GC-EAD techniques[J]. Entomological Knowledge, 2014, 0(2): 579-585
Authors:HUANG Cui-Hong  LI Jing-Jing  ZHOU Lin  YAN Feng-Ming
Affiliation:1. Shunde Polytechnic College, Guangdong 528333, China; 2. College of Plant Protection, Henan Agricultural University Zhengzhou 450002, China)
Abstract:
Electroantennography (EAG) and gas chromatography-electroantennographic detection (GC-EAD), two of the most important techniques in insect chemical ecology, play key roles in the study of insect chemoreception mechanisms, the screening of bioactive components of insect pheromones or plant allelochemicals, and so on. This paper describes technical principles, detailed operational procedures and examples of EAG and GC-EAD techniques, and discusses some key points in the application of these two techniques.
Keywords:electroantennography, EAG  gas chromatography-electroantermographic detection, GC-EAD, principle, operation procedure
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