Adventitious shoot formation and plant regeneration from Pinus pinaster Sol. ex Aiton |
| |
Authors: | Filomena Calixto M. Salomé Pais |
| |
Affiliation: | (1) Centro de Biotecnologia Vegetal, Faculdade de Ciências de Lisboa, Edifício C2 Piso 1, Campo Grande, P-1700 Lisboa, Portugal |
| |
Abstract: | Summary Pinus pinaster plants were regenerated from cotyledons excised from in vitro germinated seeds and axenically cultured on induction medium (GMD). 6-Benzyladenine (2.2 μM) induced the highest frequency of direct bud formation from cotyledons. An average of 13.1 ± 2.1 elongated shoots per cotyledon was obtained. Germination time influenced shoot induction, and the organogenic potential decreased with explant age. Cotyledons remained for 21 d on induction medium, and in order to promote adventitious shoot elongation, they were transferred to Gupta and Durzan’s DCR medium without growth regulators, containing 0.5% (wt/vol) activated charcoal and 3% (wt/vol) sucrose. Rooting was achieved by application of an indole-3-butyric acid, (396.6 μM) pulse (24 h at 4° C), followed by transfer to a sterile mixture of peat plus perlite (1:1 vol/vol). Ninety-eight to 100% of the regenerated plants were successfully acclimatized. All plants have survived after transfer to the field. |
| |
Keywords: | cotyledon micropropagation organogenesis Pinus pinaster rhizogenesis |
本文献已被 SpringerLink 等数据库收录! |
|