Modified linker-PCR primers facilitate complete sequencing of DGGE DNA fragments |
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| Authors: | O'Sullivan Louise A Webster Gordon Fry John C Parkes R John Weightman Andrew J |
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| Institution: | Cardiff School of Biosciences, Cardiff University, Main Building, Park Place, Cardiff, Wales, UK. OsullivanLA@Cardiff.ac.uk |
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| Abstract: | Modified linker-PCR primers were developed to enable complete sequencing of a DGGE band in one reaction. Commonly used bacterial and archaeal 16S rRNA gene PCR-DGGE primers were modified to contain linkers and sequencing primers. This protocol does not involve additional stages, and improves retrieval of sequence from DGGE bands by approximately 23%. |
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