Continuous-flow fast atom bombardment-mass spectrometry of permethylated oligosaccharides: a comparative study of direct mixture analysis with packed capillary column liquid chromatography-fast atom bombardment-mass spectrometry |
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| Authors: | P Boulenguer Y Leroy J M Alonso J Montreuil G Ricart C Colbert D Duquet C Dewaele B Fournet |
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| Affiliation: | Laboratoire de Chimie Biologique de l'Université des Sciences et Techniques de Lille Flandres Artois (UA du CNRS No. 217), Villeneuve d'Ascq, France. |
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| Abstract: | Conventional positive fast atom bombardment (FAB) and continuous-flow FAB analysis were carried out with permethylated lacto-N-tetraose. This latter method, a new approach, has been used to analyze a mixture of permethylated oligosaccharides by liquid chromatography-mass spectrometry (LC-MS) with a packed capillary fused-silica column and the continuous-flow probe as interface. Under these conditions, we found the continuous-flow probe to be superior to the conventional probe because its low matrix level increased the signal-to-noise ratio. The analysis of the mixture of permethylated oligosaccharide alditols obtained from hen ovomucoid by LC-MS using the continuous-flow probe as interface is described. |
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