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| 甘草酸二铵对于HCV相关性B-NHLCD25-和CD25+T细胞增殖影响研究 | |
| 引用本文： | 倪磊,姚志强,陈信义. 甘草酸二铵对于HCV相关性B-NHLCD25-和CD25+T细胞增殖影响研究[J]. 现代生物医学进展, 2010, 10(6) |
| 作者姓名： | 倪磊 姚志强 陈信义 |
| 作者单位： | 1. 北京中医药大学东直门医院肿瘤血液科,北京,100700 2. 美国东田纳西州州立大学Quillen医学院内科系感染科,美国,37614 3. 教育部、北京市重点实验室(中医内科学),北京,100700 |
| 摘 要： | 目的:本研究初步探究甘草酸二铵(Diammonium Glycyrrihizinate,DG)对HCV相关性B细胞非霍奇金淋巴瘤(B-cellnon-Hodgkin's lymphoma,B-NHL)CD25-T细胞、CD25+T细胞的免疫调控作用。方法:应用流式细胞分析仪检测HCV相关性B-NHL CD4+CD25+T细胞占CD4+T细胞的比例、CD25+细胞占总PBMC比例,并与单纯HCV感染患者、健康人检测结果相对照。应用免疫磁珠分离法(MACS)分选获得CD25-和CD25+细胞,将两者和未分选的外周血单个核细胞(peripheral blood mononuclearcell,PBMC)以CFSE标染孵育72小时后,应用流式细胞分析仪将APC CD3阳性细胞设定为门检测CD25-T细胞、未分选T细胞、CD25+T细胞的增殖情况,及甘草酸二铵干预后的CD25-T细胞、CD25+T细胞增殖情况。结果:应用流式细胞分析仪检测健康人、单纯HCV感染者和HCV相关性B-NHL患者在CD4+CD25+占总CD4+细胞比例和CD25+占总细胞比例上均呈现逐步递增的关系(分别为33.94%±2.18%,57.95%... |
| 关 键 词： | HCV 非霍奇金淋巴瘤 CD25 甘草酸二铵 |
Differential proliferation of CD25-and CD25+T cells in HCV-associated B-NHL intervened by Diammonium Glycyrrihizinate |
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| NI Lei,YAO Zhi-qiang,CHEN Xin-yi. Differential proliferation of CD25-and CD25+T cells in HCV-associated B-NHL intervened by Diammonium Glycyrrihizinate[J]. Progress in Modern Biomedicine, 2010, 10(6) | |
| Authors: | NI Lei YAO Zhi-qiang CHEN Xin-yi |
| Affiliation: | NI Lei1,YAO Zhi-qiang2,CHEN Xin-yi3 (1 Oncology , Hematology Department,Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine,Beijing 100700,China,2 Department of Internal Medicine,Division of Infectious Diseases,James H.Quillen College of Medicine,East Tennessee State University,Johnson City,TN,USA,3 Key Laboratory of the Ministry of Education , Beijing City:Internal Chinese Medicine,China) |
| Abstract: | Objectives: The study primarily explored the immune regulatory effect of Diammonium Glycyrrihizinate (DG) on HCV-associated B-cell non-Hodgkin's lymphoma (B-NHL).Methods: The percentages of CD4+CD25+ T cells in CD4+ T cells and CD25+ cells in all peripheral blood mononuclear cells (PBMCs) in patients with HCV-associated B-NHL were detected by flow cytometry in comparison with only HCV-infected patients and health donors.CD25and CD25+ cells were isolated by magnetic cell sorting (MACS).Then CD25and CD25+ cel... |
| Keywords: | HCV non-Hodgkin's lymphoma(NHL) CD25 Diammonium Glycyrrihizinate(DG) |
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