High-resolution genetic and physical mapping of the region containing the Bs2 resistance gene of pepper |
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| Authors: | T. Tai D. Dahlbeck R. E. Stall J. Peleman B. J. Staskawicz |
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| Affiliation: | (1) Department of Plant Biology, 111 Koshland Hall, University of California, Berkeley, CA 94720, U.S.A. e-mail: stask@nature.berkeley.edu, US;(2) Department of Plant Pathology, University of Florida, Gainesville, FL 32611, U.S.A., US;(3) Keygene, P.O. Box 216, NL-6700 AE Wageningen, The Netherlands, NL |
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| Abstract: | The Bs2 resistance gene of pepper confers resistance against the bacterial pathogen Xanthomonas campestris pv. vesicatoria. As a first step toward isolation of the Bs2 gene, molecular markers tightly linked to the gene were identified by randomly amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP) analysis of near-isogenic lines. Markers flanking the locus were identified and a high-resolution linkage map of the region was developed. One AFLP marker, A2, was found to cosegregate with the locus, while two others, F1 and B3, flank the locus and are within 0.6 cM. Physical mapping of the A2 and F1 markers indicates that these markers may be within 150 kb of each other. Together, these results indicate that the Bs2 region may be cloned either by chromosome walker or landing. The linked markers were also used to characterize gamma-irradiation-induced mutants at the Bs2 locus. Received: 15 January 1999 / Accepted: 11 May 1999 |
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| Keywords: | Bs2 resistance gene Pepper RAPD AFLP Positional cloning |
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