Oxylipin covered ascospores of Eremothecium coryli |
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| Authors: | NJ Leeuw JLF Kock CH Pohl AS Bareetseng OM Sebolai M Joseph CJ Strauss PJ Botes PWJ van Wyk S Nigam |
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| Institution: | (1) UNESCO MIRCEN: Industrial Biotechnology, Department of Microbial, Biochemical and Food Biotechnology, University of the Free State, 339, Bloemfontein, South Africa;(2) Centrum for Confocal and Electron Microscopy, University of the Free State, 339, Bloemfontein, South Africa;(3) Eicosanoid and Lipid Research Division, and Centre for Experimental Gynecology and Breast Research, University Medical Centre Benjamin Franklin, Free University Berlin, D-12200 Berlin, Germany |
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| Abstract: | Eremothecium coryli is known to produce intriguing spindle-shaped ascospores with long and thin whip-like appendages. Here, ultra structural
studies using scanning electron microscopy, indicate that these appendages serve to coil around themselves and around ascospores
causing spore aggregation. Furthermore, using immunofluorescence confocal laser scanning microscopy it was found that hydrophobic
3-hydroxy oxylipins cover the surfaces of these ascospores. Using gas chromatography–mass spectrometry, only the oxylipin
3-hydroxy 9:1 (a monounsaturated fatty acid containing a hydroxyl group on carbon 3) could be identified. Sequential digital
imaging suggests that oxylipin-coated spindle-shaped ascospores are released from enclosed asci probably by protruding through
an already disintegrating ascus wall. |
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| Keywords: | Ascospore Confocal laser scanning microscopy Eremothecium coryli 3-Hydroxy oxylipins Spindle-shaped Whip-like appendages |
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