| 人诱导多能干细胞与人胚胎干细胞分化过程中Oct4/Nanog 基因表达的比较 |
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| 引用本文： | 杜丽丽,林戈,卢光琇. 人诱导多能干细胞与人胚胎干细胞分化过程中Oct4/Nanog 基因表达的比较[J]. 现代生物医学进展, 2011, 11(1): 1-4 |
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| 作者姓名： | 杜丽丽 林戈 卢光琇 |
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| 作者单位： | 中南大学生殖与干细胞工程研究所,湖南,长沙,410078;人类干细胞国家工程研究中心,湖南,长沙,410078 |
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| 基金项目： | 国家973项目(2005CB522705); 国家863计划项目(2006AA02A102) |
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| 摘 要： | 目的:比较通过慢病毒方法获得的人诱导多能性干细胞(iPSCs)与人胚胎干细胞(hESCs)分化过程中全能性基因Oct4、Nanog的表达变化。方法:收集分化不同时间点的拟胚体(EBs),检测三胚层分化基因以及全能性基因Oct4/Nanog的表达,并通过畸胎瘤组织切片的荧光染色分析Oct4的表达。结果:iPSCs获得的EB中内外三胚层分化基因表达的出现明显晚于hESCs来源的EB。不同于hESCs,iPSCs悬浮培养获得的EBs在体外培养18天未见内源性Oct4、Nanog基因表达的下调。未分化的iPSCs注射严重联合免疫缺陷(SCID)小鼠培养10周后获得的畸胎瘤中仍存在Oct4阳性的细胞,但iPS-#2中明显少于iPS-#5。结论:通过慢病毒方法获得的iPSCs虽然具有向三胚层分化的能力,但在分化过程中仍维持较高水平的全能性基因Oct4、Nanog的表达。
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| 关 键 词： | 诱导性多能干细胞 胚胎干细胞 分化 拟胚体 畸胎瘤 |
Comparison of Human Induced Pluripotent Stem Cells and Oct4/NanogGene Expression in Human Embryonic Stem Cell Differentiation |
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| DU Li-li,LIN Ge,LU Guang-xiu. Comparison of Human Induced Pluripotent Stem Cells and Oct4/NanogGene Expression in Human Embryonic Stem Cell Differentiation[J]. Progress in Modern Biomedicine, 2011, 11(1): 1-4 |
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| Authors: | DU Li-li LIN Ge LU Guang-xiu |
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| Affiliation: | 1,2△(1 Institute of Reproductive & Stem Cell Engineering,Central South University,Changsha 410078; 2 National Engineering Research Center of Human Stem Cells,Changsha 410078,China) |
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| Abstract: | Objective: To compare the expression of pluripotency gene Oct4/Nanog in the differentiated human induced pluripo-tent stem cells(iPSCs) and embryonic stem cells(ESCs).Methods: The RNA of embryoid bodies(EBs) derived from undifferentiated human iPSCs and hESCs at different time of differentiation were collected.The gene expression related to three-layer differentiation and pluripotency gene Oct4/Nanog was detected by RT-PCR.The Oct4 positive cells in the teratoma derived from both human iPSCs and hESCs were ... |
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| Keywords: | Induced pluripotent stem cells Embryonic stem cells Differentiation Embryoid bodies(EBs) Teratoma |
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