| 群体感应信号分子及其抑制剂快速检测方法的建立 |
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| 引用本文： | 储卫华,刘永旺,朱卫.群体感应信号分子及其抑制剂快速检测方法的建立[J].生物技术通报,2011(3). |
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| 作者姓名： | 储卫华 刘永旺 朱卫 |
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| 作者单位： | 1. 中国药科大学生命科学与技术学院,南京,210009
2. 南京农业大学动物医学院,南京,210095 |
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| 基金项目： | 江苏省自然科学基金项目(BK2009691) |
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| 摘 要： | 细菌能自发产生、释放一些特定的信号分子,并能感知其浓度变化,调节微生物的群体行为,这一调控系统称为群体感应。细菌群体感应参与包括人类、动植物病原菌致病力在内的多种生物学功能的调节,群体感应抑制剂成为抗感染药物开发的靶点。利用紫色色杆菌(Chromobacterium violaceum)和根癌农杆菌(Agrobacterium tumefaciens)作为指示菌,建立检测高丝氨酸内酯(AHLs)及其抑制剂的简便方法。结果表明,通过平板交叉划线接种,使用指示菌能够有效地检测AHLs,并且通过薄层层析(TLC)与细菌生物感应器相结合的方法可以快速、方便地鉴定AHLs的种类;通过双层平板法观察指示菌色素产生情况,能够有效地检测群体感应信号分子AHLs抑制剂,且该方法简单易行。
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| 关 键 词： | 群体感应 N-酰基-高丝氨酸内酯 群体感应抑制剂 检测 |
Rapid Detection of Quorum Sensing Signal Molecules N-acylhomoserine Lactones and Its Inhibitors |
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| Chu Weihua,Liu Yongwang,Zhu Wei.Rapid Detection of Quorum Sensing Signal Molecules N-acylhomoserine Lactones and Its Inhibitors[J].Biotechnology Bulletin,2011(3). |
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| Authors: | Chu Weihua Liu Yongwang Zhu Wei |
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| Institution: | Chu Weihua1 Liu Yongwang2 Zhu Wei1(1School of Life Science & Technology,China Pharmaceutical University,Nanjing 210009,2College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095) |
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| Abstract: | Quorum sensing(QS)is a mechanism by which diverse microorganisms can control specific processes in response to population density.A relatively well-known form of QS among proteobacteria involves production and subsequent response to N-acylated homoserine lactones(AHLs).Quorum sensing inhibition(QSI),targeting AHL-dependent signaling,has been developed as a target for anti-infection drug.In order to detect AHL and it's inhibitors,a convenient and sensitive bioassay was developed based on the production of pi... |
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| Keywords: | Quorum sensing N-acylhomoserine lactonase Quorum sensing inhibitor Detection |
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