| 固定化酵母乙醇发酵及固化小球微生态的研究 |
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| 引用本文： | 巫小丹,徐尔尼,徐颖宣,罗玉芬,刘玉环.固定化酵母乙醇发酵及固化小球微生态的研究[J].中国生物工程杂志,2011,31(3):71-75. |
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| 作者姓名： | 巫小丹 徐尔尼 徐颖宣 罗玉芬 刘玉环 |
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| 作者单位： | 1. 南昌大学食品科学与技术国家重点实验室 南昌 330047;
2. 南昌大学高等研究院 南昌 330031;
3. 南昌大学生物质转化教育部工程技术研究中心 南昌 330047 |
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| 基金项目： | 江西省教育厅重点科技资助项目(GJJ09016) |
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| 摘 要： | 比较了SA-PVA-SiO2固定化酿酒酵母和游离酿酒酵母的乙醇发酵能力,采用批次发酵试验研究固定化酿酒酵母的发酵稳定性。结果表明,SA-PVA-SiO2固定化酿酒酵母的发酵速度比游离酿酒酵母快,发酵周期短;发酵稳定性很好,30℃,橡胶塞、90 r/min摇床培养24 h时,乙醇体积分数均在3%~3.5％之间,连续发酵14批次后,固化小球的形态依然完好,不发粘。通过扫描电镜对酿酒酵母包埋微生态环境进行了分析,图像表明固定化小球的内部环境非常有利于酵母细胞的厌氧发酵产乙醇,充分证明了SA-PVA-SiO2固定化酿酒酵母乙醇发酵的优越性。
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| 关 键 词： | 酿酒酵母 固定化 乙醇 扫描电镜 |
| 收稿时间： | 2010-09-21 |
| 修稿时间： | 2010-11-16 |
Study of Ethanol Fermentation by Immobilized Saccharomyces cerevisiae and Immobilized Microecology |
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| WU Xiao-dan,XU Er-ni,XU Ying-xuan,LUO Yu-fen,LIU Yu-huan.Study of Ethanol Fermentation by Immobilized Saccharomyces cerevisiae and Immobilized Microecology[J].China Biotechnology,2011,31(3):71-75. |
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| Authors: | WU Xiao-dan XU Er-ni XU Ying-xuan LUO Yu-fen LIU Yu-huan |
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| Institution: | WU Xiao-dan1 XU Er-ni1 XU Ying-xuan2 LUO Yu-fen1 LIU Yu-huan1,3(1 State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang 330047,China)(2 Institute for Advanced Study,Nanchang 330031,China)(3 The Engineering Research Center for Biomass Conversion,China) |
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| Abstract: | The ethanol fermentation ability of SA-PVA-SiO2 immobilized Saccharomyces cerevisiae and free S. cerevisiae was compared. In addition, batch fermentation was used to test the stability of ethanol fermentation of immobilized S. cerevisiae. The results showed that, the fermentation speed of SA-PVA-SiO2 immobilized S. cerevisiae was faster than free S. cerevisiae; the fermentation stability of the immobilized S. cerevisiae was good, the volume fraction of ethanol remained 3%~3.5 ％ when cultured at 30 ℃ for 24 hours using rubber plug and the shape of balls were still intact, not sticky afte 14 batches continuous fermentation; The internal immobilized environment was very conducive to the anaerobic fermentation to produce ethanol by SA-PVA-SiO2 immobilized S. cerevisiae. |
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| Keywords: | Saccharomyces cerevisiae Immobilized Ethanol SEM |
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