| 脊神经损伤后钠电流的变化及其离子通道机制 |
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| 引用本文： | 任鹏程 张旭东 吕海港 咎玉玲 刘怡 安丽君. 脊神经损伤后钠电流的变化及其离子通道机制[J]. 现代生物医学进展, 2011, 11(4): 677-679 |
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| 作者姓名： | 任鹏程 张旭东 吕海港 咎玉玲 刘怡 安丽君 |
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| 作者单位： | 1. 第四军医大学唐都医院全军骨肿瘤研究所,陕西,西安,710038
2. 第四军医大学唐都医院风湿免疫科,陕西,西安,710038 |
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| 摘 要： | 目的:检测脊神经切断大鼠背根节(DRG)神经元重复放电能力和钠电流的变化,并研究介导其电流变化的钠通道亚型的表达情况。方法:脊神经切断术后2～8d慢性痛大鼠模型背根节急性分离,对中等直径DRG神经元运用全细胞膜片钳技术记录神经元放电和钠电流的变化。对背根节神经元进行RT-PCR检测,分析其钠通道亚型的表达情况。结果:电流钳下,实验组DRG神经元在电流刺激下产生重复放电,而对照组神经元多诱发单个动作电位,电压钳记录发现实验组背根节神经元快钠电流和持续性钠电流幅值均明显大于对照组,PCR结果显示,Nav1.3、Nav1.7和Nav1.8通道亚型mRNA表达显著增高。结论:钠通道介导了脊神经受损模型的DRG神经元兴奋性增高,持续性钠电流可能通过调节阈下膜电位振荡的产生调节神经元兴奋性。
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| 关 键 词： | 背根节 持续性钠电流 钠通道 |
Change of Sodium Current and Its Ion Channels Mechanism AfterSpinal Nerve Injury |
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| REN Peng-cheng,ZHANG Xu-dong,LU Hai-gong,JIU Yu-ling,LIU Yi,AN Li-jun. Change of Sodium Current and Its Ion Channels Mechanism AfterSpinal Nerve Injury[J]. Progress in Modern Biomedicine, 2011, 11(4): 677-679 |
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| Authors: | REN Peng-cheng ZHANG Xu-dong LU Hai-gong JIU Yu-ling LIU Yi AN Li-jun |
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| Affiliation: | REN Peng-cheng1,ZHANG Xu-dong1,LU Hai-gong1,JIU Yu-ling1,LIU Yi1,AN Li-jun2(1 Institute of Orthopedic Oncology of PLA,Xi'an 710038,China,2 Department of Rheumatology and Immunology,Tangdu Hospital,The Fourth Military Medical University,China) |
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| Abstract: | Objective:To detect the changes of sodium influx in the transected spinal nerve rats and to investigate the expression of the sodium channel subunits.Methods:2~8d after spinal nerve transection in rats model of chronic pain,the DRG were acutely isolated.The neuron discharge and sodium influx of medium-diameter DRG neurons were detected by the whole cell recording patch clamp tech-nique.The DRG neurons,the expression of sodium channel subunits were detected by RT-PCR.Results:the repetitive firing of DRG neur... |
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| Keywords: | Dorsal root ganglia Persistent sodium current Sodium channel |
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