Expression,secretion and surface display of a human alkaline phosphatase by the ciliate <Emphasis Type="Italic">Tetrahymena thermophila</Emphasis> |
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| Authors: | Ingo Aldag Ulrike Bockau Jan Rossdorf Sven Laarmann Willem Raaben Lutz Herrmann Thomas Weide Marcus WW Hartmann |
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| Institution: | 1.Cilian AG,Münster,Germany;2.University Hospital Münster, Department of Internal Medicine D,Division of Molecular Nephrology,Münster,Germany;3.Carl Zeiss MicroImaging GmbH,G?ttingen,Germany;4.AM-Pharma Holding BV,Bunnik,The Netherlands;5.Evonik Degussa,Marl,Germany |
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| Abstract: | Background
Tetrahymena thermophila possesses many attributes that render it an attractive host for the expression of recombinant proteins. Surface proteins
from the parasites Ichthyophthirius multifiliis and Plasmodium falciparum and avian influenza virus antigen H5N1 were displayed on the cell membrane of this ciliate. Furthermore, it has been demonstrated
that T. thermophila is also able to produce a functional human DNase I. The present study investigates the heterologous expression of the functional
human intestinal alkaline phosphatase (hiAP) using T. thermophila and thereby presents a powerful tool for the optimization of the ciliate-based expression system. |
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