| QscR基因的调控对菌株M.sp.SDM11产L-丝氨酸的影响 |
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| 引用本文： | 孔庆胜,张向阳,韩晓琳,林强,董全林.QscR基因的调控对菌株M.sp.SDM11产L-丝氨酸的影响[J].生物技术通报,2011(2). |
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| 作者姓名： | 孔庆胜 张向阳 韩晓琳 林强 董全林 |
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| 作者单位： | 1. 济宁医学院,日照,276826
2. 华中科技大学,武汉,430071 |
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| 基金项目： | 山东省教育厅资助项目(J07YD16) |
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| 摘 要： | M.sp.SDM11是一株能以甲醇为唯一碳源生长的细菌,初步发酵检测发现能转化甘氨酸为L-丝氨酸。QscR基因产物是甲基营养菌中丝氨酸循环的一个转录调控关键因子,根据在GenBank中已报道的QscR基因序列(登录号:NC_012988.1)设计引物,以M.sp.SDM11的染色体DNA为模板,利用PCR扩增得到一大小为987 bp的QscR基因,将该基因克隆到广泛宿主载体pLAFR3上,在帮助质粒pRK2013的介导下,利用三亲本结合使其导入到菌株SDM11中构建重组菌株SDM12。对SDM12进一步研究发现,重组菌株中与L-丝氨酸合成相关的关键酶丝氨酸羟甲基还原酶(SHMT)的酶活比野生型菌株SDM11要低,约为野生型菌株的70%左右,另一个酶——羟基丙酮酸还原酶(HPR)的酶活力也只有野生型的75%。进一步将菌株进行产L-丝氨酸研究,结果表明,重组菌的产L-丝氨酸能力也明显降低,约为野生型菌株的67%左右。
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| 关 键 词： | QscR基因 重组菌 L-丝氨酸 |
The Influence of QscR Gene Regulation on the Production of L-serine by the Bacterial Strain M.sp.SDM11 |
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| Kong Qingsheng,Zhang Xiangyang,Han Xiaolin,Lin Qiang,Dong Quanlin.The Influence of QscR Gene Regulation on the Production of L-serine by the Bacterial Strain M.sp.SDM11[J].Biotechnology Bulletin,2011(2). |
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| Authors: | Kong Qingsheng Zhang Xiangyang Han Xiaolin Lin Qiang Dong Quanlin |
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| Institution: | Kong Qingsheng1 Zhang Xiangyang1 Han Xiaolin1 Lin Qiang2 Dong Quanlin2(1Jining Medical University,Rizhao 276826,2Huazhong University of Science and Technology,Wuhan 430071) |
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| Abstract: | M.sp.SDM11 is a bacterium living on methanol as the sole carbon source.It is found through tentative fermentation inspections to be capable of transforming glycine into L-serine.M.sp.SDM11 is a key determinant for transformation regulation in the circulation of glycine in methylotrophic bacteria.The research designed primers according to the QscR gene series reported in GenBank,took the chromosome DNA of M.sp.SDM11 as the mold,acquired a 987 bp QscR gene through PCR enlargement,cloned the gene into the broa... |
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| Keywords: | QscR gene Recombinant L-serine |
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