| 分子印迹技术应用于血清中地高辛的快速检测 |
| |
| 引用本文： | 李敏,张娟琨,张子怡,张彬彬,范婷,吴萍. 分子印迹技术应用于血清中地高辛的快速检测[J]. 生物加工过程, 2011, 9(2): 64-67. DOI: 10.3969/j.issn.1672-3678.2011.02.013 |
| |
| 作者姓名： | 李敏 张娟琨 张子怡 张彬彬 范婷 吴萍 |
| |
| 作者单位： | 1. 天津科技大学,生物工程学院,工业微生物教育部重点实验室,天津,300457
2. 天津体育学院,运动人体科学系,天津,300381 |
| |
| 基金项目： | 科技部资助项目(2009GJA10047); 天津市科委资助项目(08ZXCXSH053,09ZCZDSF04200) |
| |
| 摘 要： | 应用分子印迹的方法制备对地高辛有特异性吸附性能的印迹聚合物颗粒,再将颗粒与琼脂糖混合并固定于玻碳电极上制备成地高辛分子印迹聚合膜传感器,传感器可以特异性地结合模板分子地高辛且其电化学信号与模板浓度相关,再用它来检测血清中地高辛的含量。结果表明:分子印迹传感器具有制作简便、成本低、检测快速、特异性高、稳定性好等优点,检测下限为1.28 nmol/L,检测时间为5 min。
|
| 关 键 词： | 分子印迹传感器 地高辛 聚合物 血清 快速检测 |
Rapid detection of digoxin in serum samples by molecular imprinted biosensor |
| |
| LI Min,ZHANG Juankun,ZHANG Ziyi,ZHANG Binbin,FAN Ting,WU Ping. Rapid detection of digoxin in serum samples by molecular imprinted biosensor[J]. Chinese Journal of Bioprocess Engineering, 2011, 9(2): 64-67. DOI: 10.3969/j.issn.1672-3678.2011.02.013 |
| |
| Authors: | LI Min ZHANG Juankun ZHANG Ziyi ZHANG Binbin FAN Ting WU Ping |
| |
| Affiliation: | LI Min1,ZHANG Juankun1,ZHANG Ziyi2,ZHANG Binbin1,FAN Ting1,WU Ping1(1.Key Laboratory of Industrial Microbiology of the Ministry of Education,College of Bioengineering,Tianjin University of Science and Technology,Tianjin 300457,China,2.Department of Health and Exercise Science,Tianjin University of Sport,Tianjin 300381,China) |
| |
| Abstract: | The biosensor was prepared by molecular imprinted technique with digoxin-specific adsorption properties.Firstly the imprinted polymer particles were prepared.Then,particles and the agarose were mixed in proportion.The molecular imprinted polymer film sensor for the digoxin was fixed by the mixture on a glassy carbon electrode(GC).The sensor had specific combination of template molecule-digoxin and its electrochemical signals associated with the template concentration.Thus,it could be used to detect digoxin ... |
| |
| Keywords: | molecular imprinted sensor digoxin polymer serum rapid detection |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
