| 植物内生枯草芽孢杆菌纳豆激酶同源基因的序列分析及其在大肠杆菌中的表达 |
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| 引用本文： | 姜嵛,梁晨,姜国勇. 植物内生枯草芽孢杆菌纳豆激酶同源基因的序列分析及其在大肠杆菌中的表达[J]. 生物技术通报, 2011, 0(3) |
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| 作者姓名： | 姜嵛 梁晨 姜国勇 |
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| 作者单位： | 青岛农业大学植物基因工程研究所,青岛,266109 |
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| 基金项目： | 国家自然科学基金项目(30771440); 国家转基因重大专项课题(2008ZX08009-003) |
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| 摘 要： | 利用基因组学和生物信息学的方法,从一种植物内生枯草芽孢杆菌菌株Bs0922的基因组DNA中克隆了纳豆激酶的同源基因NK-Bs0922,长度为1 149 bp。通过重组和转化在大肠杆菌BL21(DE3)中表达了一个大小为48.0 kD的重组蛋白。
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| 关 键 词： | 内生菌 枯草芽孢杆菌 纳豆激酶 同源基因 |
Sequence Analysis of Nattokinase Homologous Gene from Endophytic Bacterial Bacillus subtilis Strain Bs0922 and Expression in E.coli |
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| Jiang Yu,Liang Chen,Jiang Guoyong. Sequence Analysis of Nattokinase Homologous Gene from Endophytic Bacterial Bacillus subtilis Strain Bs0922 and Expression in E.coli[J]. Biotechnology Bulletin, 2011, 0(3) |
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| Authors: | Jiang Yu Liang Chen Jiang Guoyong |
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| Affiliation: | Jiang Yu Liang Chen Jiang Guoyong(Institute of Plant Genetic Engineering,Qingdao Agricultural University,Qingdao 266109) |
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| Abstract: | With genomics and bio-informatics method in this report,a 1 149 bp of nattokinase homologous gene NK-Bs0922 has been cloned from a strain of B.subtilis Bs0922 which isolated within plant tissue.By means of recombination and transformation,it has been expressed a recombined protein with 48.0 kD of molecular weight in E.coli BL21(DE3). |
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| Keywords: | Endophytic bacteria Bacillus subtilis Nattokinase Homologous gene |
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