| 农杆菌介导法在橡胶树遗传转化中的应用与展望 |
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| 引用本文： | 邹智,杨礼富. 农杆菌介导法在橡胶树遗传转化中的应用与展望[J]. 生物技术通报, 2011, 0(1) |
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| 作者姓名： | 邹智 杨礼富 |
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| 作者单位： | 中国热带农业科学院橡胶研究所,农业部橡胶树生物学重点开放实验室,儋州,571737 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项(YWFZX2010-9); 公益性行业科技专项经费(nyhyzx07-033-1) |
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| 摘 要： | 综述农杆菌介导法在巴西橡胶树遗传转化中的应用进展,分析影响农杆菌转化的关键因素,如植物基因型与外植体、菌株与载体类型、菌液浓度与侵染时间、vir诱导物、筛选剂与抑菌剂、培养基的组成和附加成分等,并对提高巴西橡胶树转化效率的策略进行探讨。
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| 关 键 词： | 巴西橡胶树 根癌农杆菌 遗传转化 农杆菌介导法 |
Applications and Prospects of Agrobacterium tumefaciensmediated Transformation in Hevea brasiliensis |
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| Zou Zhi,Yang Lifu. Applications and Prospects of Agrobacterium tumefaciensmediated Transformation in Hevea brasiliensis[J]. Biotechnology Bulletin, 2011, 0(1) |
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| Authors: | Zou Zhi Yang Lifu |
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| Affiliation: | Zou Zhi Yang Lifu(Key Laboratory of Rubber Biology,Rubber Research Institute,Ministry of Agriculture,Chinese Academy of Tropical Agricultural Sciences,Danzhou 571737) |
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| Abstract: | Focusing on the progress in Agrobacterium tumefaciens-mediated transformation of Hevea brasiliensis,this review analyzed main facts influencing transformation efficiency,such as plant genotype and explant types,Agrobacterium strains and plasmid vectors,Agrobacterium density and inoculation time,virulence(vir)gene,inducing compounds,antibiotic and selectable marker,basic medium and additives.Finally,strategies to improve the efficiency are also proposed. |
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| Keywords: | Hevea brasiliensis Agrobacterium tumefaciens Genetic transformation Agrobacterium-mediated transformation |
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