| 酵母细胞中Pil1的磷酸化与细胞压力抵抗的关系 |
| |
| 引用本文： | 刘莹,王国丽,毕强,徐鸿翾,刘楠启. 酵母细胞中Pil1的磷酸化与细胞压力抵抗的关系[J]. 中国生物化学与分子生物学报, 2011, 27(3): 244-247 |
| |
| 作者姓名： | 刘莹 王国丽 毕强 徐鸿翾 刘楠启 |
| |
| 作者单位： | 中国医科大学基础医学院生物化学与分子生物学教研室; |
| |
| 摘 要： | 在外界因素处理下，细胞将启动一系列保护措施以适应各种环境改变，磷酸化调节是蛋白功能调节的主要方式. 为了探讨酵母细胞中Pil1的磷酸化与细胞压力抵抗的关系，实验应用Pil1突变细胞检测在过氧化氢或热处理后细胞的生长情况，用免疫印记法检测热处理后Pil1的表达. 结果表明,相比野生细胞，Pil1突变细胞对抗过氧化氢和热的能力强，热处理后 Pil1的磷酸化水平增高， Pil1的丝氨酸273对于其磷酸化发生至关重要.
|
| 关 键 词： | 酵母细胞中Pil1 磷酸化 压力抵抗 |
| 收稿时间： | 2010-11-10 |
Phosphorylation of Pil1 and Stress Resistance in Saccharomyces cerevisiae |
| |
| LIU Ying,WANG Guo-Li,BI Qiang,XU Hong-Xuan,LIU Nan-Qi. Phosphorylation of Pil1 and Stress Resistance in Saccharomyces cerevisiae[J]. Chinese Journal of Biochemistry and Molecular Biology, 2011, 27(3): 244-247 |
| |
| Authors: | LIU Ying WANG Guo-Li BI Qiang XU Hong-Xuan LIU Nan-Qi |
| |
| Affiliation: | LIU Ying,WANG Guo-Li,BI Qiang,XU Hong-Xuan,LIU Nan-Qi(Department of Biochemistry and Molecular Biology,Basic Medical College,China Medical University,Shenyang 110001,China) |
| |
| Abstract: | The cells can activate a series of protective signaling pathways to accommodate environmental changes.Protein phosphorylation plays an important role in regulating protein functions.To determine the relationship between Pil1 phosphorylation and stress resistance in Saccharomyces cerevisiae,a Pil1 mutant strain was treated with H2O2 or subjected to heat stress.Western blotting was used to detect Pil1 expression.The results showed that the phosphorylation of Pil1 was increased after heat stress,and the deleti... |
| |
| Keywords: | Pil1 in Saccharomyces cerevisiae phosphorylation stress resistance |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中国生物化学与分子生物学报》浏览原始摘要信息 |
|
点击此处可从《中国生物化学与分子生物学报》下载全文 |
