Surface shapes and surrounding environment analysis of single‐ and double‐stranded DNA‐binding proteins in protein‐DNA interface |
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| Authors: | Wei Wang Juan Liu Lin Sun |
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| Institution: | 1. Department of Computer Science and Technology, College of Computer and Information Engineering, Henan Normal University, Xinxiang, China;2. Laboratory of Computation Intelligence and Information Processing, Engineering Technology Research Center for Computing Intelligence and Data Mining, Henan Province, China;3. Institute of Computer Software, School of Computer, Wuhan University, Wuhan, China |
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| Abstract: | Protein‐DNA bindings are critical to many biological processes. However, the structural mechanisms underlying these interactions are not fully understood. Here, we analyzed the residues shape (peak, flat, or valley) and the surrounding environment of double‐stranded DNA‐binding proteins (DSBs) and single‐stranded DNA‐binding proteins (SSBs) in protein‐DNA interfaces. In the results, we found that the interface shapes, hydrogen bonds, and the surrounding environment present significant differences between the two kinds of proteins. Built on the investigation results, we constructed a random forest (RF) classifier to distinguish DSBs and SSBs with satisfying performance. In conclusion, we present a novel methodology to characterize protein interfaces, which will deepen our understanding of the specificity of proteins binding to ssDNA (single‐stranded DNA) or dsDNA (double‐stranded DNA). Proteins 2016; 84:979–989. © 2016 Wiley Periodicals, Inc. |
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| Keywords: | single‐strand DNA binding proteins double‐strand DNA binding proteins surface shape hydrogen bonds electrostatic charge surrounding environments |
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