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Effects of Preservatives on Viability of Cryptosporidium parvum Oocysts |
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| Authors: | A T Campbell L J Robertson and H V Smith |
| Abstract: | Potassium dichromate and formalin reduced the viability of Cryptosporidium parvum oocysts as assessed by inclusion or exclusion of 4′,6-diamidino-2-phenylindole (DAPI) and propidium iodide (PI) and excystation. Some formalin-treated oocysts containing dead sporozoites excluded PI; that this fluorogenic assay relies not solely upon exclusion of PI but also upon highlighting of sporozoite nuclei by DAPI is reiterated. |
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