High cell density culture of E. coli in a fed-batch system with dissolved oxygen as substrate feed indicator |
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Authors: | J M Cutayar D Poillon |
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Affiliation: | (1) L'Air Liquide-Centre de recherche Claude Delorme, BP 126, F 78350 Les Loges En Josas, France |
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Abstract: | Summary A fed batch system using the dissolved oxygen concentration as substrate feed indicator has been developed to perform high cell density E. coli culture. A cell density of about 110 g/l (dw) has been obtained within 12 hours with an overall growth yield of about 0.68 gX/gGlucose. |
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