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High cell density culture of E. coli in a fed-batch system with dissolved oxygen as substrate feed indicator
Authors:J M Cutayar  D Poillon
Affiliation:(1) L'Air Liquide-Centre de recherche Claude Delorme, BP 126, F 78350 Les Loges En Josas, France
Abstract:Summary A fed batch system using the dissolved oxygen concentration as substrate feed indicator has been developed to perform high cell density E. coli culture. A cell density of about 110 g/l (dw) has been obtained within 12 hours with an overall growth yield of about 0.68 gX/gGlucose.
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