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A new technique for the culture of fern gametophytes
Authors:G. E. Douglas  E. Sheffield
Affiliation:(1) Department of Cell and Structural Biology, University of Manchester, M13 9PL Manchester, UK
Abstract:Fern gametophytes initiated from spores grew successfully when immobilised in reticulate polyurethane foam in liquid shake cultures. Colonisation of this matrix was complete seven days after medium inoculation and appeared to be a completely passive process. Yield (dry weight) was greater than that from liquid grown cultures.
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