| 用于荧光辅助糖电泳寡糖标尺的构建 |
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| 引用本文： | 陈晓菁,刘曼西,黄刚良,王文涛.用于荧光辅助糖电泳寡糖标尺的构建[J].生物技术,2005,15(3):53-54. |
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| 作者姓名： | 陈晓菁 刘曼西 黄刚良 王文涛 |
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| 作者单位： | 华中科技大学生命科学与技术学院,湖北,武汉,430074 |
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| 摘 要： | 荧光辅助糖电泳(FACE)是一种简洁廉价的分离糖类方法。寡糖首先与8-氨基萘基-1，3，6-三磺酸(ANTS)反应标记，然后，ANTS标记的寡糖通过在32％丙烯酰胺-2．4％双丙烯酰胺组成的分离胶上电泳从而得以相互分离。结果表明，电泳图谱能准确反映寡糖的聚合度梯度，因而，一种具有连续聚合度的淀粉水解液的荧光标记电泳图谱可以作为荧光辅助糖电泳的分子量标尺。
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| 关 键 词： | 荧光辅助糖电泳 淀粉水解液 寡糖 聚合度 |
The Scale Establishment of Oligosaccharide Degree for Fluorophore- assisted Carbohydrate Electrophoresi |
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| CHEN Xiao-jing,LIU Man-xi,HUANG Gang-liang,WANG Wen-tao.The Scale Establishment of Oligosaccharide Degree for Fluorophore- assisted Carbohydrate Electrophoresi[J].Biotechnology,2005,15(3):53-54. |
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| Authors: | CHEN Xiao-jing LIU Man-xi HUANG Gang-liang WANG Wen-tao |
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| Abstract: | Fluorophore-assisted carbohydrate electrophoresis (FACE) is a simple and inexpensive method for separating the saccharide. The oligosaccharides were tagged with the charged fluorophore 8-aminonaphthalene-1,3,6-trisulfonate(ANTS), Saccharide-ANTS adducts were then separated from one another by electrophoresis on a 32% acrylamide, 2.4% bisacrylamide polyacrylamide gel. It indicated that the FACE can show the distribution of oligosaccharide degree of polymerzation. Therefore, The scale establishment of oligosaccharide degree of polymerzation can be established by the method. |
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| Keywords: | fluorophore-assisted carbohydrate electrophoresis oligosaccharide degree of polymerzation scale |
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