利用17个微卫星标记分析鳙鱼的遗传多样性

选用本实验室克隆的17个鳙鱼微卫星分子标记分析四川泸州和江西鄱阳湖的两个种群鳙鱼的遗传多样性及种质特性，计算和统计了杂合度、多态信息含量（PIC）、有效等位基因数、等位基因频率、遗传距离、遗传相似系数、Hardy-Weinberg平衡偏离指数等方面内容。结果表明：选择使用17个微卫星标记，其中有4个为单态标记，13个为多态标记。江西和四川鳙鱼群体每个微卫星位点的平均等位基因数分别为3.325及3.882，平均有效等位基因数分别为3.531及2.676，多态位点百分率分别为82.4及70.5， 17个微卫星标记共有等位基因71个，多态微卫星位点的PIC在0.114~0.960之间变动，平均为0.417 ，两群体位点平均观测杂合度为0.385和0.452，平均期望杂合度为0.360和0.422，两个群体间的遗传相似系数为0.897，群体间的遗传距离为0.109。

Analyzing the genetic diversity of Aristichthys nobilis in China with 17 microsatellite markers

Seventeen microsatellite markers of Aristichthys nobilis previously discovered by our lab were selected to analyze the genetic diversity and characteristics of two populations of Aristichthys nobilis from Jiangxi and Sichuan provinces.The following parameters were calculated: heterozygosity,polymorphism information content(PIC),valid allele number,allele frequency,genetic distance,genetic similarity coefficient,Hardy-Weinberg balance deflection index and so on.Results show that there are four monomorphic and 13 polymorphic markers among the 17 selected microsatellite markers.The average of allele number in each microsatellite locus of the Jiangxi population and Sichuan populations is 3.325 and 3.882,respectively;the average valid allele number is 3.531 and 2.676,respectively;and the number of total alleles of these 17 microsatellite loci is 71.The PIC of polymorphic loci varies between 0.077~0.960,and the average PIC is 0.417.The average observed heterozygosity(Ho) of two populations is 0.385 and 0.360,respectivelyand the average expected heterzygosity(He) is 0.452 and 0.422,respectively.The genetic similarity coefficient of two populations of Aristichthys nobilis is 0.897 and the genetic distance of these populations is 0.109.