Abstract: | Examination of the initial rates of enzyme activities in variousseed lots demonstrated that amylase, glutamic-pyruvic-transaminase,RNase, and glutamate decarboxylase follow the vigour profile,namely, an increase after 6 d of aging treatment followed bya decrease up to 48 d of aging time. Acid phosphatase, tetrazoliumreduction, and 14C]leucine incorporation decline directly withthe time of aging and no increase in those activities at 6 daging could be observed. Proteinase is the only enzyme foundso far that increases during accelerated aging. It is postulatedthat this enzyme may be the cause of the drop in all other enzymeactivities and therefore may play an important role in the lossof seed vigour. Examination of the components released from seeds into water,and of the rates of their leakage, led to the conclusion thatthe efflux reflects severely damaged seeds rather than vigourlessones. |