| 棉籽中游离棉酚的含量测定 |
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| 引用本文： | 黄永林,阮俊,杨雄辉,黄贤清. 棉籽中游离棉酚的含量测定[J]. 广西植物, 2001, 21(4): 371-373 |
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| 作者姓名： | 黄永林 阮俊 杨雄辉 黄贤清 |
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| 作者单位： | 中国科学院,广西植物研究所, |
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| 摘 要： | 用紫外分光光度法测定棉籽中游离棉酚的含量。脱除棉酚的棉籽乙醚提取物经石油醚和 75 %乙醇分离和纯化后 ,用 0 .5 %对氨基苯磺酸显色来检测棉籽中游离棉酚的含量 ,结果表明 ,该方法具有简便、重现性好、回收率高达 97%以上等优点 ,可作为棉籽中游离棉酚质量控制方法。
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| 关 键 词： | 棉籽 棉酚 游离棉酚 紫外分光光度法 |
| 文章编号： | 1000-3142(2001)04-0371-03 |
| 修稿时间： | 2001-02-20 |
Determination of the content of free gossypol in the cottonseeds |
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| HUANG Yong-lin,RUAN Jun,YANG Xiong-hui,HUANG Xian-qing. Determination of the content of free gossypol in the cottonseeds[J]. Guihaia, 2001, 21(4): 371-373 |
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| Authors: | HUANG Yong-lin RUAN Jun YANG Xiong-hui HUANG Xian-qing |
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| Abstract: | An ultraviolet spectrophotometry method had been developed for the determination of free gossypol in the cottonseeds. After the diethyl ether extract of degossypol cottonseeds was isolated and purified by peterdeum ether and 75% ethyl alcohol, it can be used to the determination of the content of free gossypol in the cottonseeds by revealing colour with 0.5% sulfanilic acid. It is a simple, good reproducibility and high recovery rate(over 97%)method for the quality control of free gossypol in the cottonseeds. |
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| Keywords: | cottonseed gossypol free gossypol ultraviolet spectrophotometry |
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