Simple approach for analysis of plasma oxo-, hydroxy- and dicarboxylic acids |
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Authors: | Petr Hušek Petr Matucha |
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Abstract: | Following deproteinization of plasma with organic solvents the supernatant was shaken with hexane and cation-exchange resin in an Eppendorf tube to remove fatty and amino acids and the medium was subjected to direct treatment with ethyl chloroformate under catalytic influence of pyridine. A subsequent extraction of the immediately formed ethyl esters with a drop of chloroform enabled us to subject the sample to gas chromatographic (GC) analysis. Since ketocarboxylic acids do not require a preliminary oximation the total time of sample workup and analysis takes only several minutes. |
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Keywords: | Ketocarboxylic acids Hydroxycarboxylic acids Dicarboxylic acids Carboxylic acids |
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