Assay for the determination of urinary 8-hydroxy-2′-deoxyguanosine by high-performance liquid chromatography with electrochemical detection |
| |
Authors: | D. Germadnik,A. Pilger,H.W. Rü diger |
| |
Affiliation: | Department of Occupational Medicine, University of Vienna/ Vienna General Hospital, Währinger Gürtel 18-20, A-1090 Vienna, Austria |
| |
Abstract: | A high-performance liquid chromatographic procedure with electrochemical detection is described for the determination of urinary 8-hydroxy-2′-deoxyguanosine, a major oxidative DNA lesion induced by radical forming agents. A two-step solid-phase extraction procedure was followed for extracting 8-hydroxy-2′-deoxyguanosine from human urine and the analysis was performed on a RP-18 analytical column under isocratic conditions. The limit of detection of 8-hydroxy-2′-deoxyguanosine in urine was found to be 0.9 nM. The non-invasive assay provides an indirect measurement of oxidative DNA damage. |
| |
Keywords: | 8-Hydroxy-2&prime -deoxyguanosine DNA |
本文献已被 ScienceDirect 等数据库收录! |