Salt-independent adsorption of human serum proteins on cyanocarbon gels |
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Authors: | Patrick P Berna Jerker Porath |
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Institution: | aBiochemical Separation Centre, Uppsala University, BMC, Box 577, S-751 23 UppsalaSweden;bARL Division of Biotechnology and Department of Biochemistry, University of Arizona, P.O. Box 210088, Biosciences West 350, Tucson, AZ 85721-0088, USA |
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Abstract: | Electron donor acceptor gels based on cyanocarbons have been tested for human serum protein adsorption in the absence of salt-promotion by water-structuring salt. This phenomenon was compared with a normal adsorption process in the presence of salt. The tricyanoaminopropene–divinyl sulfone–agarose displayed unusual protein adsorption properties as binding could occur both independently or dependently of the salt-promotion. The absence of hydrophobic or ionic character of the salt-independent interaction suggests an electron donor acceptor adsorption mechanism which is shown, for the first time, to occur independently of salt-promotion in aqueous solution. Study of the protein adsorption specificity showed similar protein selectivity for the fractions adsorbed in both conditions. |
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Keywords: | Electron donor acceptor gels Adsorption chromatography salt-promoted |
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