Development and validation of a capillary zone electrophoresis method for the determination of atropine, homatropine and scopolamine in ophthalmic solutions |
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Authors: | Samir Cherkaoui Lidia Mateus Philippe Christen Jean-Luc Veuthey |
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Affiliation: | Laboratory of Pharmaceutical Analytical Chemistry, University of Geneva, Boulevard d'Yvoy 20, 1211 Geneva 4, Switzerland |
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Abstract: | A capillary zone electrophoresis method is described for the simultaneous determination of atropine, homatropine and scopolamine. Successful results were obtained after optimization of the electrophoretic parameters such as buffer composition and pH. The best separation was achieved using a 100 mM Tris-phosphate running buffer at pH 7. The validation data proved that the method had the requisite selectively, reproducibility and linearity to be used for the assay of these compounds in pharmaceutical formulations. Dosage of the separate drugs in ophthalmic preparations is also presented. |
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Keywords: | Atropine Homatropine Scopolamine Tropane alkaloids |
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