Measurement of synthetic phenolic antioxidants in human tissues by high-performance liquid chromatography with coulometric electrochemical detection |
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| Authors: | L Bianchi MA Colivicchi L Della Corte M Valoti GP Sgaragli P Bechi |
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| Institution: | aDipartimento di Farmacologia Preclinica e Clinica M. Aiazzi Mancini, Università degli Studi di Firenze, Viale G.B. Morgagni 65, 50134 Firenze, Italy;bIstituto di Science Farmacologiche, Università degli Studi di Siena, Via E.S. Piccolomini 170, 53100 Siena, Italy;cClinica Chirurgica III, Università degli Studi di Firenze, V.le G.B. Morgagni 65, 50134 Firenze, Italy |
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| Abstract: | The antioxidants, 2-tert.-butyl-4-methoxyphenol (BHA) and its oxidative peroxidation product 2,2′-dihydroxy-3,3′-di-tert.-butyl-5,5′-dimethoxybiphenyl (di-BHA), 3,5-di-tert.-butyl-4-hydroxytoluene (BHT) and propyl gallate, were measured in plasma and tissue homogenates by HPLC and electrochemical detection, with a sensitivity down to 0.2 (BHA), 0.1 (di-BHA), 0.4 (BHT) and 1 (propyl gallate) ng ml−1 of plasma or tissue homogenate. The data demonstrate that in man, at the current level of exposure to dietary antioxidants, significant amounts of BHA, BHT and propyl gallate are accumulated in the omentum. Furthermore, they provide the first evidence that the peroxidase-catalysed oxidation of BHA is operative in man. |
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| Keywords: | 2-tert -Butyl-4-methoxyphenol 2 2&prime -Dihydroxy-3 3&prime -di-tert -butyl-5 5&prime -dimethoxybiphenyl 3 0-Di-tert -butyl-4-hydroxytoluene Propyl gallate Phenolic antioxidants |
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