Effects of incubation temperature on microspore callus production and plant regeneration in wheat anther cultures |
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| Authors: | Bin Huang |
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| Affiliation: | (1) Institute of Genetics, Academia Sinica, Peking, PRC;(2) Present address: Horticulture Department, Purdue University, 47907 West Lafayette, IN, USA |
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| Abstract: | Anthers of wheat cultivars Orofen and Pitic 62 were incubated for 8 days at 15, 20, 25, 30, 35 and 40°C before transfer to 25°C. Compared with anthers cultured at 25°C constantly, anthers treated at 30°C produced 40% more microspore callus and green plants in both cultivars whereas those treated at 35°C produced 2–3 fold more green plants. Treatment at 40°C was deleterious. Possible modes of action of high temperature on callus production and albinism were discussed. |
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| Keywords: | wheat (Triticum aestivum) anther culture temperature callus green plant albinism |
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