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A new method for direct detection of Listeria monocytogenes from foods by PCR.
Authors:S Makino   Y Okada     T Maruyama
Abstract:The preparation of DNA by alcohol precipitation in the presence of Na1 was used to enable the direct detection of Listeria monocytogenes in the amount of 10(3) CFU/0.5 g of sample. This procedure produces PCR-quality DNA directly from foods, such as soft cheese and minced meat.
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