棕背树蜥不同组织三种同工酶之间的差异研究

文章采用垂直板聚丙烯酰胺凝胶电泳方法,研究了分布在广东韶关地区的棕背树蜥(CalotesemmaGray)的肝脏、肌肉、心脏、脑、生殖腺五种组织酯酶(EST)、淀粉酶(AMY)和过氧化氢酶(CAT)三种同工酶,研究结果,酯酶(EST)同工酶有两条共有的酶带,Rf8除了肝脏不具备外,心脏、肌肉、脑和性腺共有,体现肝脏的特异性,性腺酶带有明显的性别差异;淀粉酶(AMY)同工酶有一条共同酶带,肝脏酶带最为复杂,其次是生殖腺和雌体心脏,五种组织中有三种组织出现雌雄差异;过氧化氢酶(CAT)有两条共有的酶带,肝脏和生殖腺的酶带复杂,而且出现明显的性别差异性,心脏、肌肉和脑酶带相似,呈现不同组织的同源性.上述果表明三种同工酶在不同的组织表现出明显的同源性和差异性,雌雄个体间同一种酶表现出性别差异。

Study on the divergence of three isozyme patterns in different tissues of Calottes emma Gray

Three kinds of isozyme,EST.AMY and POD from five tissues,liver,muscle,heart,brain and sex gland,of Calotes emma Gray collected from Shaoguan district,Guangdong province were analysed by the method of PAGE.The results show the three isozymes take on the obvious diversity and homology.The same kind of enzyme shows sexual difference between male and female.EST isozymes possess two bands in common.RF8 existed in heart,muscle,brain and sex gland but in liver that indicated the speciality of liver.The band of enzyme from sex gland showed the obvious sexual difference.AMY isozymes possess a band in common.The band of enzyme from liver is the greatest complex.Three of the five tissues presented the difference between male and female.CAT sozymes possess two bands in common.The bands of enzyme from liver and gonad are the greatest complex and they show obvious sexual difference.The bands of enzyme from heart,muscle and brain are quite similar that show the homology of different tissues.