Strand breakage in DNA by silicic acid |
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| Authors: | S G Khan R Y Rizvi S M Hadi Q Rahman |
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| Affiliation: | Industrial Toxicology Research Centre, Lucknow, India. |
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| Abstract: | The alkaline unwinding assay has been used to demonstrate the formation of single-strand breaks in DNA on treatment with silicic acid. Double-stranded DNA, containing no single-strand breaks, when incubated with increasing concentrations of silicic acid, showed the formation of an increasing number of strand breaks per molecule. Experiments on reduction of silicic acid-treated DNA with NaBH4 suggested the possibility of creation of apurinic or apyrimidinic sites. The significance of silicic acid interaction with cellular DNA during asbestos exposure is discussed. |
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