Gradual Depletion of 2,4-D in the Culture Medium for Indirect Shoot Regeneration from Leaf Explants of Camellia sinensis (L.) O. Kuntze |
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Authors: | Indra Sandal Ajay Kumar Amita Bhattacharya Madhu Sharma Adarsh Shanker Paramvir Singh Ahuja |
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Institution: | (1) Division of Biotechnology, Institute of Himalayan Bioresource Technology, Palampur, 176061, HP, India |
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Abstract: | Adventitious shoot regeneration via callus phase from in vitro leaf explants is reported for the first time in tea. Callus was obtained on Murashige and Skoog medium supplemented with
varied concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) (2.5, 5.0, 7.5 and 10.0 mg/l). Rhizogenesis was observed at
all concentrations of 2,4-D. Adventitious shoot buds developed indirectly on leaf explants after prolonged culture for 16 weeks
on medium supplemented with 10.0 mg/l 2,4-D. GC analysis of the medium and the tissues at different stages of development
showed that specific levels of 2,4-D in the tissue were responsible for morphogenesis. Shoot buds developed on rhizogenic
calli, only when 2,4-D declined to undetectable or negligible concentrations in the tissue probably due to detoxification
and metabolism. Alternatively, shoot buds could also be evoked when rhizogenic calli were transferred to medium supplemented
with low concentration of 2,4-D (1.5 mg/l). The adventitious nature of the shoots was confirmed through histological studies. |
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Keywords: | Callus Camellia sinensis 2 4-D Leaf explant Shoot regeneration |
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