Calcium dependent aggregation of marine sponge cells is provoked by leukotriene B4 and inhibited by inhibitors of arachidonic acid oxidation |
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Authors: | A M Rich G Weissmann C Anderson L Vosshall K A Haines T Humphreys P Dunham |
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Institution: | 1. New York University Medical Center, New York, NY, USA;2. Marine Biological Laboratory, Woods Hole, MA, USA;3. Syracuse University, Syracuse, NY, USA;4. Pacific Biomedical Research Center, University of Hawaii, Honolulu, HI, USA |
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Abstract: | The mechanism of agonist-induced desensitization of the beta adrenergic receptor coupled adenylate cyclase has been studied in a smooth muscle cell line, BC3H-1, which expresses both alpha and beta adrenergic receptors and nicotinic receptors. beta receptors have been investigated in intact cells using as radioligand 3HCGP-12177, an hydrophilic compound which labels only surface receptors. The treatment of BC3H-1 cells with the agonist Isoproterenol, at 37 degrees but not at 4 degrees, induced a dose dependent internalization of the beta adrenergic receptor. Agonist-induced internalization was very rapid, in the order of few minutes. beta adrenergic receptor internalization was very specific: the alpha adrenergic agonist Phenylefrine had almost no effect on beta receptor levels, while Isoproterenol treatment had no effect on the number of alpha adrenergic or nicotinic receptors expressed at the cell surface of these cells. beta adrenergic receptor internalization is probably the major mechanism responsible for catecholamine desensitization in smooth muscle cells. |
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Keywords: | MAF NDGA nordihydroguaiaretic acid NSAIDs nonsteroidal antiinflammatory drugs CMFSW calcium magnesium free sea water EDTA ethylenediamine tetracetate CTC chlorotetracycline HPLC high performance liquid chromatography DMSO dimethyl sulfoxide HETE hydroxyeicosatetraenoic acid PG prostaglandin |
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