Phosphotransfer signal transduction between two regulatory factors involved in the osmoregulated kdp operon in Escherichia coli |
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Authors: | K Nakashima A Sugiura H Momoi T Mizuno |
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Institution: | Laboratory of Microbiology, School of Agriculture, Nagoya University, Japan. |
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Abstract: | The proteins KdpD and KdpE are regulatory factors critically involved in the osmotic regulation of the kdpABC operon that is responsible for a high-affinity transport system in Escherichia coli. In this study, we obtained biochemical evidence supporting the view that the KdpD protein is a sensory protein kinase that exhibits autophosphorylation and KdpE-phosphotransfer characteristics. During the course of such studies we established a procedure for purifying the KdpE protein in large quantities. We also developed a procedure for preparing cytoplasmic membrane enriched with the KdpD protein that exhibits in vitro ability with regard to phosphorylation of KdpE protein. |
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