Construction of a new shuttle vector pSTE33 and its stabilities in Bacillus stearothermophilus,bacillus subtilis,and Escherichia coli |
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| Authors: | Issay Narumi Noriyuki Nakayama Shinya Nakamoto Toshiyuki Kimura Tadashi Yanagisawa Hiroshi Kihara |
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| Institution: | (1) Department of Agricultural Chemistry, Utsunomiya University, 350 Minemachi, Utsunomiya, 321 Tochigi, Japan;(2) NEC corporation, 4-1-1 Miyazaki, Miyamae-ku, Kawasaki, 216 Kanagawa, Japan;(3) Jichi Medical School, School of Nursing, 3311-159 Yakushiji, Minamikawachi, 329-04 Tochigi, Japan |
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| Abstract: | Summary A shuttle vector that could replicate in B. stearothermophilus, B. subtilis, and E. coli was constructed from B. stearothermophilus cryptic plasmid pSTK1, E. coli vector pUC19, and a thermostable kanamycin-resistance marker. This new vector was stably maintained in B. stearothermophilus at 67°C without selective pressure. |
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