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Reconstitution of Glyphosate Resistance from a Split 5-Enolpyruvyl Shikimate-3-Phosphate Synthase Gene in Escherichia coli and Transgenic Tobacco |
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| Authors: | Bao-Qing Dun Xu-Jing Wang Wei Lu Zhong-Lin Zhao Song-Na Hou Bao-Ming Zhang Gui-Ying Li Thomas C Evans Jr Ming-Qun Xu and Min Lin |
| Abstract: | A highly N-phosphonomethylglycine (glyphosate)-resistant Pseudomonas fluorescens G2 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) was mapped to identify potential split sites using a transposon-based linker-scanning procedure. Intein-mediated protein complementation was used to reconstitute glyphosate resistance from the genetically divided G2 EPSPS gene in Escherichia coli strain ER2799 and transgenic tobacco. |
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